For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. . 87. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. This enables the standard curve to be plotted and sample concentrations to be calculated automatically by SoftMax. 00. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. Top reading usually provides better signal-to-noise ratios for solution-based assays such as DNA quantification or protein quantification. on SpectraMax M2 fluorescence plate reader (Molecular Devices, CA, USA): excitation wavelength 320nm, emission wavelength 470nm. A microplate reader is a device used to detect chemical, biological, or physical reactions by measuring emitted light. 8 foot) 4400-0002 Power cord, 1 meter (3. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. Luminex Magpix. 47 and 0. Tristar 3 Multimode Microplate Reader. Dual monochromators allow selection of any absorbance wavelength between 200 nm and 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. 60 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Plate reader instructions Computer. 1 SoftMax Pro v4. It may be named "Pothos". g. We suggest Corning 384-well plates Cat. . The Spectramax 190 has an array of data collection modes, including kinetic, endpoint, and spectral scan modes. If a lab requires instruments to read ELISA plates, one to do fluorescence and one for luminescence, it will likely look for one instrument that can handle all these. E Models. The University of Rouen. 5. Learn how you can utilize microplate readers to drive landmark discoveries. Last lab had ours for years and never had a problem with it. The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. If you are going to read it from the bottom, you should use a clear-bottom black plate. SpectraMax M2 plate reader (Molecular Devices) was employed to measure the absorbance at 490 nm and 680 nm. 25 to 3. Type: Microplate Reader. Biotek Synergy 2 Plate Reader Multi-Mode; Related products. 314 low lamp level warning. すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. WinKQCL ® Software guides the user through the process of setting up a microplate template and running an assay to determine the amount of endotoxin in a sample solution. • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. Microplate Readers. 9000-0756. The fluorescence was measured in the SpectraMax M3 Microplate reader. Based on the SpectraMax M5e platform, this five-mode reader addresses multiple application technologies including: absorbance, fluorescenceSeller assumes all responsibility for this listing. SpectraMax M2 and use thereof is covered by issued (U. Tecan’s. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Spectramax Multi-Mode Microplate Reader Performance Specifications. We would like to show you a description here but the site won’t allow us. Appendix A. The cooled PMT detector allows high sensitivity in a wide dynamic range, even in extremely low light. 627. Standard. 7°C and the bottom to 36. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. The SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and. D 3 1. With-dye, Continual Mode Assay on a SpectraMax M2 Plate Reader. (a) Chemical structures of 1 (HADP) and its analogues 2–4. • Fluorescence Intensity (FI) • Luminescence (Lum) • Time-Resolved Fluorescence (TRF) • Fluorescence Polarization (FP) The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. Figure 2 shows a Lck kinase dilution curve obtained using the FAM-p34cdc2-derived peptide. D 47 7. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. Note: Check the Applies to section at the bottom of the page to make this is the proper driver for your plate reader. 5 pM typical (0. Instruments are configurable for the assay-specific detection modes and performance your laboratory needs. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Perkin Elmer Envision. The SpectraMax® M2 and M2e Multimode. Endpoint, kinetic, spectrum. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. $ 9,499. 0 × 107) RLU 0. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Adjusting fluorescence gain on microplate readers. 0077 OD 0. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Parameter EarlyTox Live/Dead EarlyTox Glutathione EarlyTox Caspase-3/7 R110 Read mode Fluorescence Fluorescence Fluorescence Read type Endpoint Endpoint Endpoint Wavelengths Lm1:. Multimode Reader Comparison Chart. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax ® Pro. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader Absorbance ü ü ü ü Fluorescence Intensity ü ü ü ü Luminescence ü ü ü ü Time-Resolved Fluorescence ü ü ü Fluorescence Polarization ü ü HTRF ü Upgrade Options TRF, HTRF, FP HTRF, FP HTRF N/A Plate Formats 6-, 12-, 24-, 48-, 96-, 384. 7. 次いで、ユーザーのラボにとって最も役立つ機能を有するモデルを自由に. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. This can increase throughput and efficiency, thus saving time and space in the lab. Description 1. D 4/21/06 9:53:29 AM . 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应Instrument settings used with the SpectraMax M5 Microplate Reader. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Capture flash assays with ease Expand your lab’s capabilities to include flash applications such as dual luciferase and ATP assays with the SpectraMax iD3 injector system withThis Molecular Devices SpectraMax iD5 Microplate Reader is made in Oct. 0 out of 5 $ 3,895. B. We would like to show you a description here but the site won’t allow us. Temperature variation ± 0. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Reviews. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. At different time points, the plate was removed from the incubator and loaded to the plate reader for fluorescence reading (excitation 450 nm,. High tolerance for contaminants. Similar settings will work for other SpectraMax readers with fluorescence detection. LuminescencePhotometricPerformance. Description The onboard microprocessor calculates and reports the absorbance, % transmittance, and/or fluorescence for each well of a microplate or from the cuvette port. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1 SpectraMax ABS Plus microplate reader and Absorbance plate. The plate blank’s RFU values should be subtracted from the RFUs of all samples of the plate. 00 Free shippingResults show a linear relationship between fluorescence intensity and cell number (Fig. Category 2 and 3 microplate readers use gradient heating as well. Microplate Readers Biotek Synergy 2 Plate Reader Multi-Mode. The SpectraMax iD3 reader measures absorbance, fluorescence, and luminescence. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. Measure more accurately. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. 0 out of 5 $ 7,999. On the SpectraMax iD5 reader, install the 320/100 excitation filter and 616/10 and 665/10 emission filters, and then select these filters from the Excitation and Emission dropdown wavelength menus. 0 out of 5 $ 9,995. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. a multi-detection microplate reader with two-mode cuvette port Spectramax® m2m2e user guide SpectraMax M2e user guide cover 1 1 42106 9:53:28 AM SpectraMax M2 SpectraMax…Spectramax i3 Plate Reader. In general, reading fluorescence from the top is more sensitive than reading from the bottom. We use Spectramax M5 microplate reader in our lab. As new assays are developed or yourFor TRF assays, you will need a fluorescence plate reader equipped with a time-resolved fluorometry option and a 330 nm excitation filter and emission filters capable of detecting 647 nm (620/40) and 665 nm (665/ 7. 45 s, and a 1,536-well plate in 135 s (minimum times) Interface. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. 8 Customer Reviews. In addition, the SpectraMax iD5SpectraMax Microplate Readers. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. PC software (SkanIt software) Dimensions (D x W x H) 58 x 53 x 51 cm (23 x 21 x 20 in) Weight. With exceptional signal-to-noise ratio and a lower detection limit. Next add 100 µl of workingThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. SPECTRAmax GEMINI’s wavelength choices for cutoff filters are 420, 435, 455, 475, 495, 515, 530, 550, 570, 590, 610, 630, 665 and 695 nm. The percent The percent (4) Asiaticoside reverses M2 phenotype macrophage polarization-evoked osteosarcoma cell malignant behaviour by TRAF6/NF-κB inhibition Pharmaceutical Biology December 31, 2022 Dang-ke. Flexibility to read 6- to 384-well plates. 1. Microplate Formats: 6, 12, 24, 48, 96, 384. 1 cm) Weight 27 lb. By employing dyes that specifically bind to nucleic acids, DNA/RNA concentrations can also be quantified using fluorescence detection. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). orporation. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Perkin Elmer 1420-2560 Victor X Series Plate Reader. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. 2. Refurbished. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. When the user applies a set temperature of 37°C, the top is heated to 37. Model Description. From advanced multimode luminescence, fluorescence, absorbance, BRET and FRET capabilities to dedicated microplate or single-tube luminometers, there is a GloMax® system for every lab and. Plate reader. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. In addition, the SpectraMax iD5The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. The plate is set up for reading and images are acquired according to specified parameters. The optical systems use two scanning monochromators soUnlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. The SpectraMax® i3 Multi-Mode Detection Platform for absorbance, fluorescence, and luminescence detection, and the ability to add cartridges as new assays are developed. (in addition to fluorescence imaging) on the SpectraMax i3x Multi-Mode Microplate Reader. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 0 Concentration Dynamic Range 105 RFU MT oltage ignal Adjustable PMT gain High. 67. The dual monochromator optics allow the. Plate Readers. Multimode Readers Comparison Chart. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. 00. Plate Shaking. Appendix. Our lab has a BMG labtech Pherastar FS, it is easy to use and we have never had any issue with it. 209, but in 96-well plate was 0. 315 can't find zero order. SpectraMax Glo Steady-Luc Reporter Assay Kit. View online or download Molecular devices SpectraMax M5 User Manual. Transcreener ® ADP. Butyrate (5 μM, 24h), doxorubicin (1 μM, 2h) and H 2 O 2 (2 mM. Add to Cart. D SpectraMax® M2 SpectraMax® M2e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive. The instrument will read the plate, then the relative fluorescence units (RFUs) will be displayed in the Plate section of SoftMax Pro Software. SpectraMax M2 Multi-mode Plate reader with Absorbance and Fluorescence detection, Includes computer with software, IQ validation, and warranty. These lab workhorses are used in drug discovery. Biotek FLx800 Fluorescence Microplate Reader. Plate reader set to 50 reads. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. The SoftMax Pro Software validation package and IQ/OQ validation protocols include tools for FDA 21 CFR Part 11 compliance. Therefore, ADP production is proportional to an increase in fluorescence. . For example, microplates containing different samp les can be read using the same orThe SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. BioTek’s Cytation™3, for instance, is a multimode plate reader that also functions as a high-speed automated digital fluorescence microscope with brightfield capability. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial. We therefore gathered salient. Biotek Elx800 Microplate Reader. PerkinElmer’s new EnSight Multimode Plate Reader reads label-free Corning® Epic® technology (in addition to standard labeling technologies) and is appropriate when multiple assay types are more important than speed. This reader's dual monochromators for. Deviations in boronic acid concentration were necessary when the K ARS for a boronic acid was too low (<100 M. KEY FEATURES • Use the touchscreen to easily set up methods, runSpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. Plate Shaking Linear, orbital, double-orbital Dimensions (H x W x D) 12 x 15 x 15 in. Typical settings for SpectraMax readers with fluorescence detection mode are shown. The percent (4) Asiaticoside reverses M2 phenotype macrophage polarization-evoked osteosarcoma cell malignant. Absorbance; Fluorescence (FI, TR, FP) Luminescence. Step 4: Use the Template Editor to assign wells to ‘Standards’ and ‘Unknowns’ groups (Figure 2). SpectraMax M2 and SpectraMax M2e from Molecular Devices are multi- detection microplate readers with dual-monochromators, dual-mode cuvette ports and top- and bottom-reading capability (top-reading only on SpectraMax M2). (a) Chemical structures of 1 (HADP) and its analogues 2–4. Many of the following instruments are multimode readers that, in addition to luminescence detection, can also be used to measure UV. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. Whether fluorescence, chemiluminescence, or visible detections - select the optimal plate for your assay!If your microplate reader requires an adapter for top reading (e. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by. It is particularly powerful when monitoring fast kinetic assays in a high. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Type Clear: Add to Cart. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 or 384 wells) and the "Select Specific" menu to choose the specific plate type. spectramax id5, spectramax id3 multi-mode microplate reader, spectramax id3, sof Created Date: 8/20/2020 9:11:23. Select the lab user profile. SpectraMax Microplate Readers. Perform the scan and note the wavelength that gives maximum emission. Protein assays. In the lower half of the window, left side, there is a box labeled reader. NanoQuant plate. Sale. Synergy H1 is a modular multimode microplate reader, with monochromator-based and filteru0002based optics. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. Microplate Formats: 6, 12, 24, 48, 96, 384. Third this recommendation. Sale price $9,995. SpectraMax readers are equipped with PathCheck Technology, which measures the pathlength in each well of a microplate and normalizes the absorbance. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. Conclusions. 0 out of 5 $ 2,999-20%. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. Other features and benefits include: Eliminate changing out filters. 020 OD 2. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). BMG Labtech - THERMOstar. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. When the user applies a set temperature of 37°C, the top is heated to 37. Plate reader set to 50 reads. The first step consisted of taking an initial fluorescence measurement using Molecular Devices SpectraMax® M2 fluorescence plate reader of a 96-well plate containing PET-RAFT polymerization reaction mixtures in individual wells. Intuitive software and help tools45 s, and a 1,536-well plate in 135 s (minimum times) Interface. 0) OD (500 to 1100) RFU (5. The superior optical system includes a xenon flash lamp and features an ultra-cooled photomultiplier tube (PMT) that reduces background noise for excellent sensitivity and a wide dynamic range. 1 fmol/well 384-well plate) Luminescence Specifications Wavelength Range 300 to 700 nm Dynamic Range < 6 decades Sensitivity 10 amol ATP typical (flash) Fluorescence Polarization Specifications Light Source. Multimode Readers Comparison Chart. 5%in96-and384-wellmicroplates. 0 out of 5 $ 14,499 $ 13,995-13%. )Molecular Devices SpectraMax M2 Microplate Reader. (b) Fluorescence responses of probe HADP and control compounds 2–4 (each 5 µM) towards human heparanase (HPA) (1 µg) in 40 mM NaOAc buffer (pH 5. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应 Instrument settings used with the SpectraMax M5 Microplate Reader. 3) At the bottom of the strip, click on "Show. It allows you to explore cellular pathways and protein activation and expression in one system. duAl monochromAtors With SpectraMax M2/M2e. #6590-0108) Fluorescence detection using the SpectraMax iD5 reader’s monochromators for wavelength selection was compared to that using a filter pair specific for the Alexa594 tracer. The method is based on. • Flexible design for future expansion • User upgradable in < 2 minutes • High speed detection for up to 1536-well microplates • Excellent. Refurbished. In simplest terms, a plate reader measures and records a signal produced from the wells of a multiwell plate. Brand: MOLECULAR DEVICES. Perkin Elmer EnVision 2104 Multilabel Plate Reader, Perkin Elmer, Envision Plate Reader. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. for the plate. Fluorescence Microplate Reader Comparison Chart. It is also extremely flexible and can measure samples held in the 96, 384, 1536 and other. 0 × 107) RLU 0. Traditional microplate readers perform mostly biochemical assays—measuring how much light passes through or is emitted by a sample. SpectraMax ABS Plus SpectraTest ABS2 v1. 7. The SPECTRAmax GEMINI XS Microplate Spectrofluorometer from Molecular Devices is a microplate reader capable of detecting signal from either fluorescence or luminescence based assays. The microplate reader will read the plate and the relative fluorescence units will be displayed in the Plate section. Reading Capabilities: cuvette or microplate. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. The flexibility of a high end multimode plate reader with environmental control. It does not work as expected. as a fluorescence blank. The SpectraTest absorbance test plate (Abs-1) can be used by VersaMax, SpectraMax, & M2 readers. The SpectraMax Plus 384 Microplate Reader for. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. Do NOT use the front panel Ref button in fluorescence mode cuvette reads. It also. The SpectraMax microplate readers support a wide variety of assay categories including . Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). CA$14,500. SpectraMax Quant dsDNA Assay Kits. To avoid these issues and provide the best possible dynamic range between the highest and the lowest measurement values of your assay, fluorescence gain is typically adjusted on the sample with the expected highest signal output (e. 1 New Reader Support 1. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. 306 plate air cal fail,low light. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). A solution for low volume applications. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. Molecular Devices SpectraMax M2 Microplate Reader. g. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. spr SpectraMax iD5 SpectraTest ABS2 v1. 1) A maximal fluorescence intensity of 177 ± 3 RFU was observed at the highest cell concentration (Table 1). Settings for SpectraMax iD3 Multi-Mode Microplate Reader. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. The IC50s by cell rounding and LysoTracker assays were 0. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western. D 59 A. Luminescence, and Fluorescence microplate re aders—providing extensive data calculation. Detection modalities include absorbance (UV-Vis Abs) and. . Using a black. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13Fluorescence Microplate Readers. The fluorometric. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. Top detection is available for fluorescence detection on the SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2e. Microplate Reader, For Lab. spr * SpectraMax i3(x) SpectraTest ABS2 v1. The kit includes everything you need: reagents, buffers and a microplate. Highly selective for dsDNA over ssDNA or RNA. Fully tested with validations and warranty. 67. TRF as Secondary Detection Mode: TRF is supported as a secondary detection mode by these readers: Gemini XS, EM, XPS, M2, & FlexStation. Main Menu Toggle. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing a single Fig 2: Enzymatic response of synthetic compounds. The Molecular Devices SpectraMax M2 Microplate Reader is a multidetection microplate reader with dualmonochromators, dual-mode cuvette ports, and top-reading capability. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization. The cuvette must be in place when you read the plate. Optimizing Time-Resolved Fluorescence Read Mode The SpectraMax M2 enables you to read Time-Resolved Fluorescence assays from the top of a plate. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The pre-configured protocols can be selected for each assay and. SpectraMax M2 Readers provide for easy conversion and optimization of very-low-throughput to medium-high-throughput assays, faster, more precise results and reagent savings. When connecting for the first time to either the SpectraMax L reader or StakMax system. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. Microplate Readers Include the M3, M4, M5, and M5. A. 0. 0 out of 5 $ 9,995 $ 9,495. The SpectraMax® ABS Plus Microplate Reader can run . SpectraTest FL1 Fluorescence Validation . 1. 0 out of 5. 67. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. Used. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 67. The SpectraMax. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. The SpectraMax i3 Multi-Mode Plate Reader enables characterization of proteins by measuring intrinsic tryptophan fluorescence. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 2. Model: SpectraMax M2. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard plate reader applications, protecting your initial investment. Like all SpectraMax® Readers, the new M3 and M4 readers leverage patented technologies to deliver added value to users. USING OLIGREEN REAGENT IN GEMINI XS, GEMINI EM AND SPECTRAMAX M2 MICROPLATE READERS Step 6: Add 100 µL of each concentration of oligonucleotide to the wells of the microplate in triplicate. begin reading microplates with seven mouse clicks. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell. 1. VANTAstar™ Flexible Multi-mode Microplate Reader. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF. Also refer to this Multimode Reader Comparison Chart. Item Description FrontPanelOperation SinglewavelengthAbsorbance,%Transmittance,Fluorescence readingofthecuvette(ortesttube) UsingSoftMaxProSoftware ExpressdataasAbsorbance. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Page 28 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 4. Our absorbance plate readers feature our PathCheck Sensor. 7°C and the bottom to 36. Microplate readers’ standard detection modes. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Plate chamber design of the SpectraMax category 1 microplate readers. The following general steps are taken with our absorbance and fluorescence readers. On the SpectraMax i3x reader, HTRF (cartridge) should be selected as the Optical Configuration. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. L evices evices orporation. Gemini XPS Fluorescence Microplate Reader . 020 OD 2. Add to Cart. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). Optimized for fluorescence microplate readers. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. SpectraMax M2. Bottom Monochromator: Fluorescein 2. Compact and tunable absorbance microplate readers DATA SHEET. The plate reader optics are tunable for absorbance. 25 to 3.